1. Guarantee the pure culture of the strain
For strains that are not contaminated by miscellaneous bacteria, do not use different strains of the same edible fungus species for mixed or close connection culture.
 
2. Control the number of subcultures
Strictly control the number of subcultures to reduce mechanical damage and ensure the vitality of the strains.
 
3. Appropriate cryogenic preservation of strains
The low-temperature strains are stored at 4°C, and the high-temperature strains are stored at 16°C, which is beneficial to preserve the vitality of the mycelium.
 
4. Avoid multiple passages in a single medium
Different types of medium along with reasonable generations, mother species, original species, and production species are conducive to improving the viability of strains and maintaining excellent traits.
 
5. The bacteria should not be used for a long time
Overage strains will age, and aging and degeneration are organically connected, and strains with weak vitality are prone to degeneration.
 
6. The strains should be rejuvenated regularly
It is carried out under conditions such as suitable temperature, suitable pH, sufficient oxygen, proper diffused light, and no bacteria culture.
 
7. Annual Spore Isolation
Sexual reproduction is used to discover excellent strains, and tissue separation is used to consolidate the genetic characteristics of excellent strains.