In the production practice of shiitake mushrooms, hericium and fungus, the secondary bagging technology is applied (that is, a film bag is added when the mushroom tube is out of the stove, so that the mushroom tube in the bag does not directly contact the ambient air) for production, which can effectively solve the problem of the mushroom tube. The problem of cross-infection during the production process keeps the survival rate of the strain above 97%. The technology is now introduced as follows:
Preparation of the outer bag. The outer bag should be a low-pressure polyethylene film bag with a circumference of 2 cm greater than that of the inner bag, a length of 5 cm longer than that of the inner bag, and a thickness of 0.2 silk, with one end knotted and sealed for later use.
Bagging in time. When the barrel goes out of the stove after high-temperature sterilization, cover it with an outer bag while taking it out, and tighten the mouth of the bag and move it into the incubation room to lower the barrel temperature below 25°C. Note: After the barrel is out of the stove, it should be quickly put into the outer bag to prevent it from being in contact with the external environment for a long time and infecting with bacteria.
Inoculation operation. During inoculation, untie the outer bag in the inoculation room or the inoculation box, push to the closed side, pull 4/5, and then inoculate, immediately put the outer bag and tighten it after inoculation.
Take off the outer bag in due course. Pay attention to the temperature of the culture room during the culture process, take off the outer bag when the mycelium of the tube is about 80%, and manage it according to conventional production methods.