1. Nutritional factors. Flammulina velutipes is one of the saprophytic fungi, and the nutrients needed for growth and development can only be obtained from the saprophytic substrate by the mycelium. For Flammulina velutipes to grow well, sufficient and stable nitrogen, some inorganic salts, and decomposed substances containing lignin and cellulose must be continuously supplied. The growth of Flammulina velutipes requires a certain amount of nitrogen to supply the growth of fruiting bodies, which is the same as that of other plants. Without nitrogen, the fruiting bodies of Flammulina velutipes cannot grow.
Flammulina velutipes need wood containing lignin and cellulose as a carbon source for growth. When the mycelium of Flammulina velutipes has a weak ability to decompose cellulose, lignin, etc. in wood, if it is cultivated with wood without accumulation and fermentation, most of them are will fail.Through experiments, discarded cottonseed husks, piled sawdust, corncob piled up after crushing, and various stale weeds and leaves are all good raw materials for cultivating Flammulina velutipes. Phosphorus, potassium and magnesium are indispensable and important elements in the growth of Flammulina velutipes. These inorganic salts should be supplemented appropriately, such as potassium dihydrogen phosphate, magnesium sulfate, calcium sulfate, iron sulfate, etc. Adding, these are important factors affecting the high quality and high yield of Flammulina velutipes. 
2. Temperature. For Flammulina velutipes to grow well, suitable temperature is essential. It can be said that temperature is a very important factor affecting the high quality and high yield of Flammulina velutipes. The hyphae and fruiting bodies of Flammulina velutipes require different temperatures. The most suitable temperature for the growth of mycelium is 22-26 degrees, and the development of fruiting bodies cannot normally grow to good quality Flammulina velutipes at this temperature. According to experiments, in the growth stage of the fruiting body of Flammulina velutipes, when the temperature exceeds 18 degrees, there will be brown spots on the mushroom cap of Flammulina velutipes, resulting in a very poor quality finished product. When the temperature is too low, the differentiation and occurrence of mushroom buds are seriously affected, which is one of the reasons why some friends have a large number of whisker-like mushrooms when planting Flammulina velutipes, which affects the commercial value of Flammulina velutipes. The most suitable temperature for the growth of Flammulina velutipes fruit body is 12~15 degrees, 10 degrees different from the temperature suitable for mycelium growth. When the fruiting body grows, under the conditions of 11-15 degrees, the formation of the fruiting body only takes nearly two weeks. Therefore, the difference in temperature is an important factor affecting the growth of Flammulina velutipes.
3. Moisture. Water is the source of life, and all living things on the earth are inseparable from water. The same is true for Flammulina velutipes. Appropriate water is the nutrient element required for the occurrence and growth of fruiting bodies. It must be absorbed under certain water conditions. Transport, to promote the value of mycelium, to achieve the purpose of fruit body growth. Flammulina velutipes has strong drought tolerance and is one of the mushrooms that likes humidity. The humidity in the cultivation room should not be too high. During the planting process, some friends controlled the humidity to more than 85%~90%, resulting in bacterial contamination. The humidity of the culture room should be controlled at about 70%, which is conducive to the growth and development of the mycelium in the early stage. The moisture in the medium should preferably be controlled at 60%~65%. Increase to between 85% and 95%, which is conducive to the growth of fruiting bodies.
4. Air. The freshness of the air is a factor that affects the growth of Flammulina velutipes. Flammulina velutipes are aerobic fungi with good ventilation and sufficient oxygen in the fresh air, and the caps are well developed. When the carbon dioxide content exceeds 1%, the growth of Flammulina velutipes is basically stagnant. Therefore, keeping the air fresh has a great impact on the growth and development of Flammulina velutipes.
5. pH value. The pH value that Flammulina velutipes likes is relatively wide, the most suitable pH value for mycelium growth is about 6, and the most suitable pH value for fruit body growth is 5
6. Light. Flammulina velutipes mycelium and cap have different requirements for light in the growth process. Mycelium prefers darkness during growth, but does not grow well in the presence of light. When fruit body grows, under dark conditions without light, no fungus will grow. The cover only has a long mushroom stalk, and the color is light, and most of the mushroom cap and mushroom stalk are light yellow or milky white. In the actual planting process, the light can be increased or decreased according to the needs to make the Flammulina velutipes reach a higher quality state.